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    Human Methylated & Non-Methylated (WGA) DNA Set #D5013

    產品品牌:Zymo Research
    產品貨號:D5013
    產品規格:5μg/20μl
    產品價格:咨詢
    資料下載:點擊下載

    產品介紹

    Product Introduction:The Human WGA Methylated & Non-methylated DNA Set consists of two control DNAs (non-methylated and methylated) along with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation, EZ DNA Methylation-Gold, EZ DNA Methylation-Direct, and EZ DNA Methylation-Lightning kits from Zymo Research to assess the efficiency of bisulfite-mediated conversion of DNA. The Human WGA Methylated & Non-methylated DNA Set is generated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CG dinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.

    Highlights:

      ●  Purified, non-methylated and methylated human DNA for use as negative and positive control in methylation                   detection applications.

      ●  Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.

      ●  Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP). Primers included with               the full set are designed to amplify non-methylated, methylated, and mixed methylated copies of the Ras                          association (RalGDS/AF-6) domain family member 1 (RASSF1) gene following bisulfite conversion.

    Concentration:250 ng/μl in buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)

    Sample Source:Whole genome amplified DNA from HCT116 DKO cells. Human WGA Methylated DNA is enzymatically methylated by M.SssI methyltransferase.

    Sample Storage:≤ -20°C



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